Serial Sci-Fi: Awakening by Linda Harley, Chapter 1

Check back every Friday for another chapter of Awakening, Nuelda Book 1, by Linda Harley.

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AWAKENING
Nuelda Book 1

Chapter 1

White penetrating light blasted into Anna’s eyes. Her entire body felt numb from the cold and she could just barely move her toes. She lay in a glass casket, her body suspended in the air. The pressure in the casket started to change as she drifted to its bottom. The smell of rust and sweat swept into the casket as the lid popped open to release her back into civilization.

The smooth face of a man came into her field of vision. “Don’t try to move Anna,” the man’s soft voice purred in her ears. She tried to nod, but none of her muscles responded.

Anna closed her eyes and tried to remember why she was here. It felt like just moments ago that she had been hugging her mom and dad and telling them goodnight. They had promised her that they would wake her once a cure was found for her cancer, but that they would wait no more than 3 years before bringing her out of cryostasis. How much time had passed? Days, weeks, months or years?

“Try to wiggle your toes Anna,” the man instructed and she obeyed. “Very good. Now flex your foot.”

Slowly and painfully, feeling returned to her body. Her bones ached as she tried to sit up in the casket that stood in the center of the return room. Rusty cables hung from the ceiling and were connected like a spider’s web to her casket. Behind the dirty glass wall stood a bank of computers, with one red-haired smooth faced woman operating the controls.

The man that had woken her stood next to the casket holding out a tattered wool robe. He helped her out of the casket and hung the robe on her scrawny frame. The robe enveloped her into its warmth but she nearly collapsed under its weight. She would have fallen but the man placed his arm around her shoulder and held her upright. “Come, you must try to walk. Your legs and body will remember what they’re supposed to do better if you move around.”

Anna hesitantly took one step and then another. Soon she was able to stand on her own, albeit with shaky legs. Finally she mustered up the courage to try and speak, but it came out as a horse croak. The man brought her a glass of water, which she drank thirstily. “Where are my parents?”

“They’re not here,” the man replied.

Anna fought back the tears that were pushing their way to the surface. “You’re lying. They promised that they’d be here.”

“I’m sorry but they’re not,” the man repeated more gently.

“Where are they?”

“I don’t know.”

Anna took a deep quivering breath. It felt good to breathe on her own again, even though the air smelled of rust and grease. Her bones complained at the abuse she was doling out on them. A stool stood at a decrepit workbench. She slowly sagged onto it, not knowing what to make of it all. “What’s your name?”

“My name is Nick. Would you like something to eat?”

Anna nodded and Nick produced a banana, a tomato and another glass of water. Slowly Anna reached for the banana and peeled it. Breaking off small pieces at a time she ate it, stopping now and then for a sip of water. Nick had disappeared to talk with the woman in the control room, leaving her to work on her snack in solitude.

When she finished, Anna left the banana peel on the table feeling guilty. Her mom would have a fit if she just left her rubbish lying around, but she could not see a trashcan anywhere. When Nick returned, he told her not to worry, that he would take care of it.

Nick came to her side and helped her to get moving once more. “How do you feel?”

“Sore,” Anna murmured. “My bones ache and my body feels numb.”

Nick led her into an elevator and pressed a button labeled Medical Ward. “Some of that is due to being in cryostasis for so long. The rest is due to your lymphoma cancer. But then you should be used to that.”

“How long have I been in cryo?”

“Some time.”

“How long Nick?”

“Look, there is a lot that I must tell you, but I’m not going to yet.”

Anna stopped and stared at him incredulously.

“I still have to treat your cancer. In order for the treatment to work swiftly you need to be in a calm state, like you are now, or we won’t have enough time with you. Do you understand?”

Anna shook her head. “I gather that whatever you have to tell me is not going to make me feel better, so why not just tell me?”

Nick sighed as the doors to the elevator opened. He led her over to a bed where he helped her to remove the robe and slip into a night dress and then under the covers.

Anna gripped Nick’s wrist. “Just tell me, please.”

Nick bent over and kissed her on her forehead. “Later, child.”

Anna bristled at being called a child by an adult. She was nearly 14, and knew more than they thought she did. They always spoke in whispers around her about her cancer, thinking she was not smart enough to understand them. But she understood every word, which was why she also knew that her only choice to live had been to go into cryo until a cure was found. Now there was a cure, but where were her parents? Were they watching her from an observation room while they administered her cure? She hoped that they were, and that all of this was normal. But then, why would Nick not tell her anything?

Nick came over and placed a calm hand on her shoulder. “Take a deep breath Anna. Everything is going to be all right. I promise.” Nick raised a needle and injected some medicine into her arm.

No matter how many times the doctors poked her with needles, she always flinched whether it hurt or not.

Anna felt herself drifting to sleep. She reached out and Nick took her hand. “Nick, please, just tell me how long was I in cryo?”

Nick cocked his head to the side, “158 years.”

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  Timothy Durham was convicted of rape when a test showed his DNA genotype matched the DNA recovered from the crime scene. Eye witnesses testified that Durham was in a different state at the time of the incident, but he wasn’t exonerated from his 3000 year sentence until follow up DNA analysis showed that the preliminary forensic analysis used in the trial was misinterpreted (Thomas et al.). DNA testing serves an invaluable part in our criminal justice system, but it comes with complications and bias that can lead to wrongful convictions and misinformed juries.  

Background:

Forensic genetics aims to identify the origin of a biological sample. It can be traced back to Karl Landsteiner who discovered human ABO blood groups. With blood groups, suspects could be eliminated based on their blood type. For example, if a suspect had type A blood and there was type B blood left at the scene by the perpetrator, the investigators could rule out the suspect. Unfortunately, the reverse didn’t work. If the suspect and perpetrator both had the same blood type, it can’t be concluded that the suspect was guilty. Blood identifiers evolved into 8 distinct categories which improved the ability to eliminate suspects, but could only conclude that (if the system matched) it might be the perpetrator or this person might be related to the perpetrator (Jobling and Gill). The field evolved in the 1980s with the discovery of Minisatellites, lines of repetitive DNA that don’t encode proteins. Minisatellite patterns in DNA create what we know today as the DNA fingerprint, and it has a much higher accuracy rate than previous methods (Augustyn et al.). Around the same time, a breakthrough for victims of sexual assault was discovered. In the case of assault, bodily fluids of both the victim and the assaulter can get mixed. A process called differential lysis was created which could selectively increase the concentration of sperm in a fluid sample thereby allowing for accurate DNA testing (Jobling and Gill). Forensics evolved to use single-locus probe testing after several years because of its ease of interpretation. It looked at specific minisatellites that had high variability between subjects. This was the first DNA-based method to be used in a criminal investigation. Two murder homicides from several years apart were connected using DNA evidence. The evidence was then used to exonerate an innocent man, and the guilty party was found when he tried to evade mandatory DNA testing for all men in the small town. His guilt was then reinforced when the DNA profiles matched, and he gave a confession (Jobling and Gill). Finding DNA at a crime scene doesn’t guarantee that you’ll find a murderer. There are issues with non-pure samples and limited samples that make DNA work difficult, and accreditation of facilities and new techniques and take time (Jobling and Gill).  

Subjectivity of DNA Interpretation:

In a 2009 publication, the National Academy of Science discusses the possibility for subjective influence in lab based forensics. The interpretation bias happens in complex cases where, for example, there could be a mixture of DNA. A study published in 2011 looked specifically at this issue. A gang rape happened in Georgia, and the mixture in DNA from the case was used to prosecute one suspect after another suspect took a plea bargain to testify against him. With DNA mixtures, there are three possible conclusions: suspect excluded, suspect cannot be excluded, and inconclusive. If the suspect is excluded, it means that there’s little to no possibility that his DNA is part of the sample. If the suspect cannot be excluded, it means that there’s a possibility that his DNA is part of the sample. In the trial, the DNA examiners who knew the context of the case concluded that the suspect being prosecuted could not be excluded. The 2011 study gave the DNA evidence from this case and the DNA profiles of the suspects to 17 forensic examiners. These examiners had no context of the case, and they all worked independently. Of the 17, only 1 came to the same conclusion (suspect cannot be excluded) as the examiners who knew the context of the case. Twelve of the 17 concluded that the suspect who was prosecuted and convicted had no relation to the DNA found in the sample. Not only does this show that subjectivity occurs due to the context of the case, but the 17 examiners came up with different findings based on their past experiences meaning that not all DNA analysis is objective. Some organizations like the Scientific Working Group on DNA Analysis Methods say that quantitative statistical evidence should be presented with every categorical conclusion, but the US doesn’t require this (Dror and Hampikian).

False Positives:

There are two categories that cause wrongful identification in a DNA report: coincidental matches and false positives. Coincidental matches are DNA matches between two unrelated people. The probability of these matches is about 1 to 500,000 for white individuals and about 1 to 350,000 for black individuals. This small probability of coincidental matches makes people feel confident in DNA evidence. False positives, on the other hand, are matches between DNA and test DNA that result from issues in the forensics lab. There have been multiple cases of forensics labs accidentally switching victim and suspected rapist DNA which lead to convictions that were later overturned when the lab realized the error. There are also cases of misreading or misunderstanding a sample. In a case from 1993, a man was convicted of rape because someone interpreted a mixed sample of victim and perpetrator DNA as an individual sample. The conviction was again overturned. To prevent false convictions, retesting DNA is allowed, but it’s not an efficient solution. Many people are pushing for statistical information on the probability of false positives, so that the jurors can make accurate judgements based on DNA evidence (Thomas et al.).  

Conclusion:

DNA analysis is a useful tool for the criminal justice system, but subjectivity can be an issue in forensic testing. To combat wrongful convictions, there are organizations like The Innocence Project who use DNA testing to free the innocent, but reformed forensic testing standards should be put in place to prevent subjectivity and to ensure juries are correctly informed during trials. https://www.innocenceproject.org/    

Citation:

Augustyn , Adam, et al. “DNA Finerprinting.” Encyclopedia Britannica , 26 Apr. 2018, www.britannica.com/science/DNA-fingerprinting#ref153186. Dror, Itiel E., and Greg Hampikian. “Subjectivity and Bias in Forensic DNA Mixture Interpretation.” Science & Justice, vol. 51, no. 4, 2011, pp. 204–208., doi:10.1016/j.scijus.2011.08.004. Jobling, Mark A., and Peter Gill. “Encoded Evidence: DNA in Forensic Analysis.” Nature Reviews: Genetics, vol. 5, no. 10, Oct. 2004, pp. 739–751., doi:10.1038/nrg1455. Thompson, William C, et al. “How the Probability of a False Positive Affects the Value of DNA Evidence.” Journal of Forensic Sciences, vol. 48, no. 1, Jan. 2003, pp. 1–8., doi:10.1520/jfs2001171.